HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by MITCHELL, L. A. Articles by ROSENKE, K. A. Search for Related Content PubMed PubMed Citation Articles by MITCHELL, L. A. Articles by ROSENKE, K. A. Agricola Articles by MITCHELL, L. A. Articles by ROSENKE, K. A.

Lymphocyte recognition of human parvovirus B19 non-structural (NS1) protein: associations with occurrence of acute and chronic arthropathy?

Department of Pathology, University of British Columbia, BC Research Institute for Children's and Women's Health, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada

Corresponding author: Dr L.A. Jonsen (Mitchell). Present address: Goldyne Savad Institute of Gene Therapy, Hadessah Hospital, P.O.B. 12000 Jerusalem 91120, Israel.

Received 15 Feb. 2000; revised version received 12 Oct. 2000; accepted 5 Nov. 2000.

Abstract

Immune recognition of recombinant parvovirus B19 non-structural (rNS1) protein was studied by immunoblot and lymphoproliferative assays in blood from the following B19 seropositive groups: B19 infected (n=14), B19 exposed but non-infected (n=16), other illness with rash (n=3), chronic arthropathy of unknown aetiology (n=4) and healthy controls (n=7). Sera from 11 B19 seronegative subjects were also studied. Sera collected at initial diagnosis or at the time of accidental B19 exposure in pregnancy were tested for NS1 antibody and evidence of B19 DNA by nested PCR. Follow-up specimens were obtained 3–12 months later for serological, PCR and proliferation studies. B19 DNA was detected sporadically in early specimens and in one follow-up specimen from a subject who developed chronic arthropathy after B19 infection. There was no correlation with development of arthropathy. NS1-specific IgG was detected in early sera from B19-infected and exposed subjects but to a lesser degree in follow-up specimens, and in only one healthy control serum. No correlation with the presence of NS1-specific antibodies was found with development of acute or chronic arthropathy. Although lymphocyte proliferation in response to stimulation with rNS1 in vitro occurred at a higher frequency in patients who developed acute and chronic joint manifestations after B19 infection, suggesting an association with this outcome, NS1-reactive lymphocytes were also found in three B19 seronegative patients, two of whom had recently been exposed to B19 but had no illness. Hence, immune recognition of NS1 may be more indicative of recent infection with, or exposure to, parvovirus B19 than associated with development of arthropathy as previously reported.

This article has been cited by other articles:

				V. Kasprowicz, A. Isa, T. Tolfvenstam, K. Jeffery, P. Bowness, and P. Klenerman Tracking of Peptide-Specific CD4+ T-Cell Responses after an Acute Resolving Viral Infection: a Study of Parvovirus B19 J. Virol., November 15, 2006; 80(22): 11209 - 11217. [Abstract] [Full Text] [PDF]

				A. Corcoran and S. Doyle Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19 J. Med. Microbiol., June 1, 2004; 53(6): 459 - 475. [Abstract] [Full Text] [PDF]

				J Dembinski, A M Eis-Hubinger, J Maar, R Schild, and P Bartmann Long term follow up of serostatus after maternofetal parvovirus B19 infection Arch. Dis. Child., March 1, 2003; 88(3): 219 - 221. [Abstract] [Full Text] [PDF]

				E. D. Heegaard and K. E. Brown Human Parvovirus B19 Clin. Microbiol. Rev., July 1, 2002; 15(3): 485 - 505. [Abstract] [Full Text] [PDF]