HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by COONEY, M.A. Articles by PALOMBO, E.A. Search for Related Content PubMed PubMed Citation Articles by COONEY, M.A. Articles by PALOMBO, E.A. Agricola Articles by COONEY, M.A. Articles by PALOMBO, E.A.

Characterisation and phylogenetic analysis of the VP7 proteins of serotype G6 and G8 human rotaviruses

M.A. COONEY, R.J. GORRELL and E.A. PALOMBO

Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Flemington Road, Parkville, Victoria 3052 and School of Engineering and Science, Swinburne University of Technology, PO Box 218 Hawthorn, Victoria 3122, Australia

Corresponding author: Dr E. A. Palombo (e-mail: epalombo{at}swin.edu.au).

Received 25 September 2000; accepted 16 Oct. 2000.

Abstract

Serotype G6 and G8 rotaviruses are rarely found in man and may have originated in animals. Human serotype G6 and G8 rotaviruses, isolated from hospitalised children at various locations in Australia, were characterised. Deduced amino acid sequences of the major neutralising antigen, V7, showed significant identity to the cognate proteins of prototype human and bovine G6 and G8 viruses, respectively, and the strains reacted with G6 and G8 serotype-specific neutralising monoclonal antibodies, respectively, in an enzyme immunoassay. The VP4 type was determined as P[14] for all strains tested. Phylogenetic analysis of these and other human and bovine VP7 sequences suggested that a single inter-species transmission event, possibly from cattle, may have led to the emergence of G6 viruses in man. In contrast, the exchange of genes between human and bovine G8 viruses may have occurred on more than one occasion, or these genes may have originated in a different host.

This article has been cited by other articles:

				S. Ghosh, V. Varghese, S. Samajdar, M. Sinha, T. N. Naik, and N. Kobayashi Evidence for Bovine Origin of VP4 and VP7 Genes of Human Group A Rotavirus G6P[14] and G10P[14] Strains J. Clin. Microbiol., August 1, 2007; 45(8): 2751 - 2753. [Full Text] [PDF]

				J. Matthijnssens, M. Rahman, X. Yang, T. Delbeke, I. Arijs, J.-P. Kabue, J.-J. T. Muyembe, and M. Van Ranst G8 Rotavirus Strains Isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup. J. Clin. Microbiol., May 1, 2006; 44(5): 1801 - 1809. [Abstract] [Full Text] [PDF]

				R. van der Heide, M. P. G. Koopmans, N. Shekary, D. J. Houwers, Y. T. H. P. van Duynhoven, and W. H. M. van der Poel Molecular Characterizations of Human and Animal Group A Rotaviruses in The Netherlands J. Clin. Microbiol., February 1, 2005; 43(2): 669 - 675. [Abstract] [Full Text] [PDF]

				K. Banyai, V. Martella, F. Jakab, B. Melegh, and G. Szucs Sequencing and Phylogenetic Analysis of Human Genotype P[6] Rotavirus Strains Detected in Hungary Provides Evidence for Genetic Heterogeneity within the P[6] VP4 Gene J. Clin. Microbiol., September 1, 2004; 42(9): 4338 - 4343. [Abstract] [Full Text] [PDF]

				V. Martella, M. Ciarlet, A. Pratelli, S. Arista, V. Terio, G. Elia, A. Cavalli, M. Gentile, N. Decaro, G. Greco, et al. Molecular Analysis of the VP7, VP4, VP6, NSP4, and NSP5/6 Genes of a Buffalo Rotavirus Strain: Identification of the Rare P[3] Rhesus Rotavirus-Like VP4 Gene Allele J. Clin. Microbiol., December 1, 2003; 41(12): 5665 - 5675. [Abstract] [Full Text] [PDF]

				M. Rahman, K. De Leener, T. Goegebuer, E. Wollants, I. Van der Donck, L. V. Hoovels, and M. Van Ranst Genetic Characterization of a Novel, Naturally Occurring Recombinant Human G6P[6] Rotavirus J. Clin. Microbiol., May 1, 2003; 41(5): 2088 - 2095. [Abstract] [Full Text] [PDF]