| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
MOLECULAR DIAGNOSIS |
Institut für Hygiene und Laboratoriumsmedizin, Klinikum Krefeld, Lutherplatz 40, 47805 Krefeld, Germany
Corresponding author: Professor C.H. Wirsing von König (e-mail: wvk_hyg{at}klinikum-krefeld.de).
Received 16 June 2000; revised version received 6 Oct. 2000; accepted 8 Nov. 2000.
Abstract
A real-time PCR assay based on the TaqMan® technology was developed for the detection of Bordetella pertussis and B. parapertussis in clinical samples. The assay was evaluated with 182 specimens from 153 patients with and without symptoms of pertussis. The analytical sensitivity ranged from 0.1 to 10 cfu for B. pertussis and B. parapertussis, respectively, and diagnostic sensitivity was 94.1% when culture was used as a reference. No sample from a patient without symptoms of pertussis was positive in PCR. Twenty-four of 28 patients who were negative by culture and positive by PCR assay met the CDC clinical case definition for pertussis; the remaining four patients had paroxysms of shorter duration. Intra- and inter-assay variation were <5% and results were available within 4 h.
This article has been cited by other articles:
|
|
 |
|
  V. Caro, N. Guiso, C. Alberti, S. Liguori, C. Burucoa, G. Couetdic, F. Doucet-Populaire, A. Ferroni, S. Papin-Gibaud, F. Grattard, et al. Proficiency Program for Real-Time PCR Diagnosis of Bordetella pertussis Infections in French Hospital Laboratories and at the French National Reference Center for Whooping Cough and other Bordetelloses J. Clin. Microbiol., October 1, 2009; 47(10): 3197 - 3203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Guthrie, C. Seah, S. Brown, P. Tang, F. Jamieson, and S. J. Drews Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay J. Clin. Microbiol., November 1, 2008; 46(11): 3798 - 3799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. S. Probert, J. Ely, K. Schrader, J. Atwell, A. Nossoff, and S. Kwan Identification and Evaluation of New Target Sequences for Specific Detection of Bordetella pertussis by Real-Time PCR J. Clin. Microbiol., October 1, 2008; 46(10): 3228 - 3231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lassmann, M. Poetschke, B. Ninteretse, S. Issifou, S. Winkler, P. G. Kremsner, W. Graninger, and P. Apfalter Community-acquired Pneumonia in Children in Lambarene, Gabon Am J Trop Med Hyg, July 1, 2008; 79(1): 109 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Espy, J. R. Uhl, L. M. Sloan, S. P. Buckwalter, M. F. Jones, E. A. Vetter, J. D. C. Yao, N. L. Wengenack, J. E. Rosenblatt, F. R. Cockerill III, et al. Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing Clin. Microbiol. Rev., January 1, 2006; 19(1): 165 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Riffelmann, C. H. Wirsing von Konig, V. Caro, N. Guiso, and for the Pertussis PCR Consensus Group Nucleic Acid Amplification Tests for Diagnosis of Bordetella Infections J. Clin. Microbiol., October 1, 2005; 43(10): 4925 - 4929. [Full Text] [PDF]
|
|
|
|
|
|
G. Muyldermans, O. Soetens, M. Antoine, S. Bruisten, B. Vincart, F. Doucet-Populaire, N. K. Fry, P. Olcen, J. M. Scheftel, J. M. Senterre, et al. External Quality Assessment for Molecular Detection of Bordetella pertussis in European Laboratories J. Clin. Microbiol., January 1, 2005; 43(1): 30 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kosters, U. Reischl, J. Schmetz, M. Riffelmann, and C. H. Wirsing von Konig Real-Time LightCycler PCR for Detection and Discrimination of Bordetella pertussis and Bordetella parapertussis J. Clin. Microbiol., May 1, 2002; 40(5): 1719 - 1722. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | J MED MICROBIOL | MICROBIOLOGY | J GEN VIROL | ALL SGM JOURNALS |
