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J. Med. Microbiol. -- Vol. 50 (2001), 415-420
© 2001 Society for General Microbiology
ISSN 0022-2615


MOLECULAR EPIDEMIOLOGY

An attempt to identify the evolutionary origin of a novel serotype of Salmonella enterica isolated from harbour porpoises

D.C. OLD, P.B. CRICHTON, A. TAYLOR and H. MATHER*

Medical Microbiology, Ninewells Hospital and Medical School, Dundee DD1 9SY and *Scottish Salmonella Reference Laboratory, Stobhill Hospital, Glasgow G21 3UW

Corresponding author: Dr D.C. Old (e-mail: davido{at}tuht.scot.nhs.uk).

Received 29 Sept. 2000; accepted 8 Nov. 2000.

Abstract

The isolation since 1991 of a new serotype of Salmonella enterica (antigenic formula 4,12:a:-) from harbour porpoises (Phocoena phocoena) at post-mortem examination raised the question of its evolutionary origin. Representative strains of S. enterica serotype 4,12:a:- and strains of eight other serotypes of serogroup O4 with phase-1 flagellar antigen H ‘a’ were examined by EcoRI ribotyping, IS200 fingerprinting and PCR-based profiling. Statistical analysis of results of multiple typing showed that strains of Salmonella serotype 4,12:a:- were genetically distant from those of antigenically similar salmonella serotypes, none of which seemed likely to be the progenitor of the ‘porpoise’ serotype.







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