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J. Med. Microbiol. -- Vol. 50 (2001), 320-329
© 2001 Society for General Microbiology
ISSN 0022-2615

BACTERIAL PATHOGENICITY

Involvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines

MARIA A. GUZMAN-MURILLO, EDUARDO RUIZ-BUSTOS, BOW HO* and FELIPE ASCENCIO{dagger}

Center for Biological Research, Marine Pathology Unit, La Paz, BCS 23000, Mexico and *Department of Microbiology, National University of Singapore, Singapore 117597

{dagger}Corresponding author: Dr F. Ascencio (e-mail: micv4@ nus.edu.sg). Present address: Department of Microbiology, National University of Singapore, MD4/A, Science Drive 2, Singapore 117597.

Received 8 May 2000; revised version received 22 Aug. 2000; accepted 11 Sept. 2000.

Abstract

To determine whether Helicobacter pylori heparan sulphate-binding proteins (HSBPs) are involved in the adherence of H. pylori to HeLa and Kato III cells, monolayers were pre-incubated with various preparations and concentrations of H. pylori HSBPs at 37°C, washed and then challenged with bacteria. HSBPs did not prevent but enhanced H. pylori adherence. However, challenging cultured cells with H. pylori previously incubated with rabbit anti-HSBP IgG resulted in significant inhibition of bacterial adherence. These data demonstrate that the extracellular HSBP plays an important role in promoting H. pylori attachment to Kato III and HeLa S3 cells, that adhesion of H. pylori to Kato III and HeLa S3 cells is promoted by the presence of the 71.5-kDa extracellular HSBP and that rabbit polyclonal antibodies against this HSBP can inhibit adhesion of H. pylori to the cultured cell lines and detach cell-bound H. pylori.




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