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J. Med. Microbiol. -- Vol. 50 (2001), 1100-1104
© 2001 Society for General Microbiology
ISSN 0022-2615

DIAGNOSTIC MICROBIOLOGY

Comparison of PCR, capture ELISA and immunoblotting for detection of Toxoplasma gondii in infected mice

J. HAFID*,{dagger}, P. FLORI*, H. RABERIN* and R. TRAN MANH SUNG*

*Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Faculté de Médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02, France and {dagger}Unité d'Immunologie et de Physiologie, Département de Biologie, Faculté des Sciences et Techniques, Avenue A. El Khattabi, BP 618, 40000 Marrakech, Morocco

Corresponding author: Dr J. Hafid (e-mail: jamal.hafid{at}univ-st-etienne.fr).

Received 2 Feb. 2001; revised version received 6 June 2001; accepted 7 June 2001.

Abstract

PCR was compared with capture ELISA and immunoblotting for the detection of Toxoplasma gondii in sera of acutely infected mice. One hundred animals were inoculated intraperitoneally with 5000 trophozoites of RH strain and five of them were killed every 3 h from 3 h to 21 h post infection (p.i.), and every day from day 1 to day 7 p.i.. No assay detected the parasite from 3 h p.i. to 15 h p.i. PCR was the most sensitive assay and detected the T. gondii from 18 h p.i., whereas the other assays detected it only from day 1.




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P. FLORI, J. HAFID, T. BOURLET, H. RABERIN, C. GENIN, and R. TRAN MANH SUNG
Experimental model of congenital toxoplasmosis in guinea-pigs: use of quantitative and qualitative PCR for the study of maternofetal transmission
J. Med. Microbiol., October 1, 2002; 51(10): 871 - 878.
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