| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CLINICAL MICROBIOLOGY |
Department of Oral Surgery, Medicine and Pathology and *PHLS Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN
Corresponding author: Dr M. A. O. Lewis (e-mail: lewismao{at}cardiff.ac.uk).
Received 9 Aug. 1999; revised version received 17 Oct. 1999; accepted 12 Nov. 1999.
Abstract
Gram-positive anaerobic cocci (GPAC) are isolated from approximately one quarter of all infections involving anaerobic bacteria. However, studies of the significance of this group of pathogens have been hindered by an inadequate taxonomy and the lack of a valid identification scheme. In the present study, a phenotypic scheme for the identification of ‘butyrate-producing’ GPAC based on the analysis of volatile fatty acid profiles by gas-liquid chromatography, biochemical profiles (including the use of the rapid ID 32 A commercial kit) and carbohydrate fermentation reactions, was evaluated. The identity of 68 clinical isolates of GPAC was determined by application of the scheme published by Murdoch. The scheme was found to be easy to apply and only four of the test isolates could not be readily assigned to a species or well-defined group. The species most frequently identified in the test collection were Peptostreptoccoccus vaginalis, P. tetradius and the ßGAL group. A large number of strains was assigned to the heterogeneous ‘prevotii/tetradius’ group. Some species regarded as being restricted to particular clinical sites were shown to be more widespread than previously thought. The clinical source of the isolates did not show any consistent correlation with species identity.
This article has been cited by other articles:
|
|
 |
|
  K. Imai, K. Ochiai, and T. Okamoto Reactivation of Latent HIV-1 Infection by the Periodontopathic Bacterium Porphyromonas gingivalis Involves Histone Modification J. Immunol., March 15, 2009; 182(6): 3688 - 3695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Riggio and A. Lennon Identification of Oral Peptostreptococcus Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes J. Clin. Microbiol., September 1, 2003; 41(9): 4475 - 4479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Song, C. Liu, M. McTeague, A. Vu, J. Y. Liu, and S. M. Finegold Rapid identification of Gram-positive anaerobic coccal species originally classified in the genus Peptostreptococcus by multiplex PCR assays using genus- and species-specific primers Microbiology, July 1, 2003; 149(7): 1719 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.P. Riggio and A. Lennon Specific PCR detection of Peptostreptococcus magnus J. Med. Microbiol., April 1, 2003; 52(4): 309 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.P. RIGGIO and A. LENNON Development of a PCR assay specific for Peptostreptococcus anaerobius J. Med. Microbiol., December 1, 2002; 51(12): 1097 - 1101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.E. HILL, C.E. DAVIES, M.J. WILSON, P. STEPHENS, M.A. O. LEWIS, V. HALL, J. BRAZIER, and D.W. THOMAS. Heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16S-23S intergenic ribosomal RNA polymorphisms J. Med. Microbiol., November 1, 2002; 51(11): 949 - 957. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | J MED MICROBIOL | MICROBIOLOGY | J GEN VIROL | ALL SGM JOURNALS |
