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Adhesion of Salmonella enterica var Enteritidis strains lacking fimbriae and flagella to rat ileal explants cultured at the air interface or submerged in tissue culture medium

J.M. C. ROBERTSON, G. GRANT, E. ALLEN-VERCOE^*,, M.J. WOODWARD^*, A. PUSZTAI and H.J. FLINT

Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB and *Bacteriology Department, Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey KT15 3NB

Corresponding author: J. M. C. Robertson (email: jmr{at}rri.sari.ac.uk). Current address: Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4 OJG.

Received 17 Sept. 1999; revised version accepted 29 Jan. 2000.

Abstract

Rat ileal air interface and submerged explant models were developed and used to compare the adhesion of Salmonella enterica var Enteritidis wild-type strains with that of their isogenic single and multiple deletion mutants. The modified strains studied were defective for fimbriae, flagella, motility or chemotaxis and binding was assessed on tissues with and without an intact mucus layer. A multiple afimbriate/aflagellate (fim^-/fla^-) strain, a fimbriate but aflagellate (fla^-) strain and a fimbriate/flagellate but non-motile (mot^-) strain bound significantly less extensively to the explants than the corresponding wild-type strains. With the submerged explant model this difference was evident in tissues with or without a mucus layer, whereas in the air interface model it was observed only in tissues with an intact mucus layer. A smooth swimming chemotaxis-defective (che^-) strain and single or multiple afimbriate strains bound to explants as well as their corresponding wild-type strain. This suggests that under the present experimental conditions fimbriae were not essential for attachment of S. enterica var Enteritidis to rat ileal explants. However, the possession of active flagella did appear to be an important factor in enabling salmonellae to penetrate the gastrointestinal mucus layer and attach specifically to epithelial cells.

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