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Enhancement of hepatocyte growth factor (scatter factor) production by human gingival fibroblasts in culture stimulated with Porphyromonas gingivalis fimbriae

A. SUGIYAMA^*,, T. OGAWA, Y. DAIKUHARA and H. TAKADA^*

*Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Department of Oral Microbiology, Asahi University School of Dentistry, Gifu 501-0296 and Department of Biochemistry, Kagoshima University Dental School, Kagoshima 890-8544, Japan

Corresponding author: Professor H. Takada (e-mail: dent-ht @mail.cc.tohoku.ac.jp). Present address: Department of Preventive Dentistry, School of Dentistry, University of Tokushima, Tokushima 770-8504, Japan.

Received 25 March 1999; revised manuscript received 17 June 1999; accepted 14 Aug. 1999.

Abstract

Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a motogen, mitogen and morphogen produced by mesenchymal cells that mainly acts on epithelial cells and is involved in osteoclast stimulation. This study examined the possible enhanced production of HGF/SF by human gingival fibroblasts upon stimulation with killed cells of Porphyromonas gingivalis strain 381 and its representative bioactive cellular components, fimbriae and lipopolysaccharide (LPS). P. gingivalis whole cells enhanced the production of HGF/SF detected by ELISA in culture supernates of the fibroblasts. Fimbriae prepared from P. gingivalis exhibited powerful HGF/SF-inducing activity in a concentration-dependent manner, with peak activity observed at 100–200 µg/ml. The fimbriae-induced HGF/SF mRNA expression by the cells was also detected by reverse transcription-PCR. P. gingivalis LPS exhibited weak HGF/SF-inducing activity. The study also examined the HGF/SF-inducing activity of seven synthetic peptides corresponding to the segments of P. gingivalis fimbrial subunit protein. The peptides of residues 282–301 and 302–321, which exhibited antagonistic effects against P. gingivalis fimbriae-binding to human gingival fibroblasts in a previous study, showed weak activity, whereas other non-antagonistic peptides showed no significant activity. These findings indicated that P. gingivalis fimbriae enhanced production of HGF/SF by human gingival fibroblasts, whereas synthetic peptide segments of fimbrial subunit protein were not sufficient to exert the activity.

This article has been cited by other articles:

				A. Uehara, K. Muramoto, T. Imamura, K. Nakayama, J. Potempa, J. Travis, S. Sugawara, and H. Takada Arginine-Specific Gingipains from Porphyromonas gingivalis Stimulate Production of Hepatocyte Growth Factor (Scatter Factor) through Protease-Activated Receptors in Human Gingival Fibroblasts in Culture J. Immunol., November 1, 2005; 175(9): 6076 - 6084. [Abstract] [Full Text] [PDF]