HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by MORAES, S.R. Articles by DOMINGUES, R.M. C. P. Search for Related Content PubMed PubMed Citation Articles by MORAES, S.R. Articles by DOMINGUES, R.M. C. P. Agricola Articles by MORAES, S.R. Articles by DOMINGUES, R.M. C. P.

Use of rep-PCR to define genetic relatedness among Bacteroides fragilis strains

S.R. MORAES, R.B. GONÇALVES^*, C. MOUTON, L. SELDIN, M.C. S. FERREIRA and R.M. C. P. DOMINGUES

Instituto de Microbiologia Prof. Paulo de Góes, UFRJ, Rio de Janeiro, Brasil *Faculdade de Odontologia de Piracicaba/UNICAMP, Brasil and Faculté de Medicine Dentaire, Universite Laval, Quebec, Canada

Corresponding author: Professor R. M. C. P. Domingues (e-mail: IMMMREG{at}microbio.UFRJ.br).

Received 17 Feb. 1999; revised version received 10 Aug. 1999; accepted 13 Aug. 1999.

Abstract

Bacteroides fragilis, a component of the normal flora and an important anaerobic pathogen in non-intestinal endogenous infections, has recently been associated with enteric diseases. In this study, 41 B. fragilis strains were analysed in relation to their genetic diversity. This collection included two reference strains (ATCC 23745 and 25285), 20 isolates from non-intestinal infections, six from intestinal infections, five from intestinal microflora and eight from an aquatic environment. The fingerprints were generated by using two repetitive sequences (REP and ERIC) as primers to PCR (rep-PCR). A dendrogram was obtained with the Taxotron® Program. Three clusters (threshold genotypes I, II and III) were observed when the genetic distance was 0.30. These results confirm previous data found regarding the genotypical diversity of B. fragilis.

This article has been cited by other articles:

				S. Lofmark, C. Jernberg, J. K. Jansson, and C. Edlund Clindamycin-induced enrichment and long-term persistence of resistant Bacteroides spp. and resistance genes J. Antimicrob. Chemother., December 1, 2006; 58(6): 1160 - 1167. [Abstract] [Full Text] [PDF]