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J. Med. Microbiol. -- Vol. 49 (2000), 29-35
© 2000 Society for General Microbiology
ISSN 0022-2615


MYCOLOGY

Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions

KOICHI MAKIMURA, YOSHIKO TAMURA, MICHINARI KUDO, KATSUHISA UCHIDA, HIUGA SAITO and HIDEYO YAMAGUCHI

Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan

Corresponding author: Dr K. Makimura (e-mail: makimura{at}main.teikyo-u.ac.jp).

Received 19 Jan. 1999; accepted 18 May 1999.

Abstract

This study demonstrated the application of internal transcribed spacer 1 (ITS1) ribosomal DNA sequences to the species identification and strain typing of 28 standard strains and 46 clinical isolates of the genus Malassezia. The size of ITS1 regions ranged from 162 to 266 bp. Members of the genus Malassezia (M. pachydermatis, M. furfur, M. sympodialis, M. globosa, M. obtusa, M. restricta and M. slooffiae) were classified into seven ITS1-homologous groups and 22 ITS1-identical, individual groups. The 46 clinical isolates of lipophilic Malassezia spp. were identified as belonging to just three ITS1-homologous groups, i.e., M. furfur (19 strains: 11 from pityriasis versicolor, 4 from seborrhoeic dermatitis and 4 from atopic dermatitis). M. sympodialis (22 strains: 7 from pityriasis versicolor, 3 from seborrhoeic dermatitis, 1 from atopic dermatitis and 11 from healthy controls) and M. slooffiae (five strains: three from chronic otitis media and two from healthy controls).




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