J Med Microbiol NEW Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, T.
Right arrow Articles by Samaranayake, L. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, T.
Right arrow Articles by Samaranayake, L. P.
Agricola
Right arrow Articles by Wu, T.
Right arrow Articles by Samaranayake, L. P.

The Journal of Medical Microbiology, Vol 48, Issue 8 711-720, Copyright © 1999 by Society for General Microbiology

JOURNAL ARTICLE

The expression of secreted aspartyl proteinases of Candida species in human whole saliva

T. Wu and L. P. Samaranayake
Oral Bio-science Laboratories, Faculty of Dentistry, University of Hong Kong, Hong Kong.

The expression of secreted aspartyl proteinases (Saps) by clinical isolates of Candida albicans, C. tropicalis and C. parapsilosis in human saliva supplemented with glucose and in a proteinase-inducing medium (YCB-BSA), was investigated. Also, yeast growth, pH fluctuation and total protein concentration of the saliva cultures during incubation were measured. Sap expression was assessed by evaluating the enzyme activity as well as the antigen concentration. Saps were expressed well in human saliva supplemented with glucose by all three Candida species, although greater expressions was found in YCB-BSA medium. C. albicans isolates were significantly more proteolytic than the non-albicans isolates. In general, for all three species, the rate of yeast growth, pH fluctuation and percentage reduction of total salivary protein concentration concurred with the degree of expression of Saps. These data strongly suggest that Saps of C. albicans, C. tropicalis and C. parapsilosis may play an active role in the progression of oral candidoses, particularly with regard to the abundance of low pH microenvironments in the oral cavity, which are regularly replenished with dietary carbohydrates.


This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
J. R. Naglik, S. J. Challacombe, and B. Hube
Candida albicans Secreted Aspartyl Proteinases in Virulence and Pathogenesis
Microbiol. Mol. Biol. Rev., September 1, 2003; 67(3): 400 - 428.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
P. K. Mukherjee, J. Chandra, D. M. Kuhn, and M. A. Ghannoum
Differential expression of Candida albicans phospholipase B (PLB1) under various environmental and physiological conditions
Microbiology, January 1, 2003; 149(1): 261 - 267.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
M. SCHALLER, E. JANUSCHKE, C. SCHACKERT, B. WOERLE, and H. C. KORTING
Different isoforms of secreted aspartyl proteinases (Sap) are expressed by Candida albicans during oral and cutaneous candidosis in vivo
J. Med. Microbiol., August 1, 2001; 50(8): 743 - 747.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1999 Society for General Microbiology.