The Journal of Medical Microbiology, Vol 48, Issue 7 657-662, Copyright © 1999 by Society for General Microbiology

JOURNAL ARTICLE

Analysis of fliC variation among clinical isolates of Burkholderia cepacia

C. Winstanley, B. A. Hales, J. A. Morgan, M. J. Gallagher, S. D. Puthucheary, M. F. Cisse and C. A. Hart
Department of Biomedical Sciences, University of Bradford, West Yorkshire. C.Winstanley@bradford.ac.uk

PCR and restriction fragment length polymorphism (RFLP) typing of flagellin genes (fliC) from 57 clinical isolates of Burkholderia cepacia indicated that only type 11 flagellins were present. Twenty-two isolates previously identified as the epidemic UK cystic fibrosis strain were indistinguishable by this method, as were 11 isolates from a pseudo-outbreak in Senegal. Other clinical isolates, including 19 from disparate sources in Malaysia, were separated into nine fliC RFLP groups, exhibiting a large degree of divergence. When isolates were indistinguishable by fliC genotyping, their similarity was confirmed by whole genome macro-restriction analysis with pulsed-field gel electrophoresis following XbaI digestion. The variation in fliC sequences of B. cepacia was far greater than that with B. pseudomallei, supporting the view that 'B. cepacia', as currently defined, may comprise several different genomic species.

This article has been cited by other articles:

				M. G. Detsika, J. E. Corkill, M. Magalhaes, K. J. Glendinning, C. A. Hart, and C. Winstanley Molecular Typing of, and Distribution of Genetic Markers among, Burkholderia cepacia Complex Isolates from Brazil J. Clin. Microbiol., September 1, 2003; 41(9): 4148 - 4153. [Abstract] [Full Text] [PDF]

				S. C. M. Miller, J. J. LiPuma, and J. L. Parke Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments Appl. Envir. Microbiol., August 1, 2002; 68(8): 3750 - 3758. [Abstract] [Full Text] [PDF]

				C. WINSTANLEY, M. G. DETSIKA, K. J. GLENDINNING, Y. N. PARSONS, and C. A. HART Flagellin gene PCR-RFLP analysis of a panel of strains from the Burkholderia cepacia complex J. Med. Microbiol., August 1, 2001; 50(8): 728 - 731. [Abstract] [Full Text] [PDF]

				A. Tasteyre, T. Karjalainen, V. Avesani, M. Delmée, A. Collignon, P. Bourlioux, and M.-C. Barc Molecular Characterization of fliD Gene Encoding Flagellar Cap and Its Expression among Clostridium difficile Isolates from Different Serogroups J. Clin. Microbiol., March 1, 2001; 39(3): 1178 - 1183. [Abstract] [Full Text]

				C. WINSTANLEY, A. SHINA, S. DAWSON, R.M. GASKELL, and C.A. HART Variation in Bordetella bronchiseptica flaA does not correlate with typing by macro-restriction analysis by pulsed-field gel electrophoresis J. Med. Microbiol., March 1, 2001; 50(3): 255 - 260. [Abstract] [Full Text] [PDF]

				A. Tasteyre, T. Karjalainen, V. Avesani, M. Delmée, A. Collignon, P. Bourlioux, and M.-C. Barc Phenotypic and Genotypic Diversity of the Flagellin Gene (fliC) among Clostridium difficile Isolates from Different Serogroups J. Clin. Microbiol., September 1, 2000; 38(9): 3179 - 3186. [Abstract] [Full Text]