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The Journal of Medical Microbiology, Vol 48, Issue 5 495-499, Copyright © 1999 by Society for General Microbiology

JOURNAL ARTICLE

Lectin typing of methicillin-resistant Staphylococcus aureus

A. Munoz, O. Alvarez, B. Alonso and J. Llovo
Department of Microbiology and Parasitology, Faculty of Biology, University of Santiago de Compostela, Spain. mpangels@uscmail.usc.es

This study reports the patterns of agglutination of 77 clinical isolates of methicillin-resistant Staphylococcus aureus by 32 commercially available lectins. Cell suspensions were not pre-treated. Each isolate was cultured on three media: Columbia blood agar, trypticase-soy agar and Chapman Stone agar. The lectins agglutinating each isolate varied widely depending on culture medium; only five isolates were agglutinated by the same set of lectins regardless of the culture medium used. Lectin typing could be a useful epidemiological tool, but it is necessary to standardise assay conditions (notably culture medium) to enable meaningful comparison of the results produced by different research groups or centres.


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H. ANNUK, S. O. HYNES, S. HIRMO, M. MIKELSAAR, and T. WADSTROM
Characterisation and differentiation of lactobacilli by lectin typing
J. Med. Microbiol., December 1, 2001; 50(12): 1069 - 1074.
[Abstract] [Full Text] [PDF]


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