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The Journal of Medical Microbiology, Vol 48, Issue 2 205-209, Copyright © 1999 by Society for General Microbiology


JOURNAL ARTICLE

Clonal structure of Providencia alcalifaciens strains isolated from diarrhoeal stools in Sao Paulo, Brazil

B. E. Guth, K. Irino and L. R. Trabulsi
Department of Microbiology, Immunology and Parasitology, Universidade Federal de Sao Paulo - Escola Paulista de Medicina, Brazil. becguth.dmip@epm.br

Clonal analysis based on ribotyping demonstrated that Providencia alcalifaciens strains isolated mainly from diarrhoeal stools in Sao Paulo, Brazil, were clustered into two main groups. Eleven distinct ribotype patterns were identified with ClAI, EcoRV and MluI restriction endonucleases. P. alcalifaciens strains with invasive properties were of two ribotype patterns that differed from those identified among non-invasive strains. The ribotyping results confirmed that P. alcalifaciens strains associated with diarrhoeal disease in Sao Paulo represent distinct groups of strains. Although the invasive strains were isolated from different patients over an extended period they were clustered into two genetically related clones, which seemed to be distributed endemically in the population studied.


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A. B.R. Vieira, I. H.J. Koh, and B. E.C. Guth
Providencia alcalifaciens strains translocate from the gastrointestinal tract and are resistant to lytic activity of serum complement
J. Med. Microbiol., August 1, 2003; 52(8): 633 - 636.
[Abstract] [Full Text] [PDF]


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M. SOBREIRA, N. C. LEAL, M. MAGALHAES, B. E. C. GUTH, and A. M. P. ALMEIDA
Molecular analysis of clinical isolates of Providencia alcalifaciens
J. Med. Microbiol., January 1, 2001; 50(1): 29 - 34.
[Abstract] [Full Text] [PDF]




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