J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dutta, S.
Right arrow Articles by Manna, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dutta, S.
Right arrow Articles by Manna, B.
Agricola
Right arrow Articles by Dutta, S.
Right arrow Articles by Manna, B.

The Journal of Medical Microbiology, Vol 48, Issue 11 1011-1016, Copyright © 1999 by Society for General Microbiology

JOURNAL ARTICLE

Use of PCR to identify enteroaggregative Escherichia coli as an important cause of acute diarrhoea among children living in Calcutta, India

S. Dutta, S. Pal, S. Chakrabarti, P. Dutta and B. Manna
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.

The importance of enteroaggregative Escherichia coli (EAggEC) as a possible aetiological agent of acute diarrhoea among children in Calcutta, India, was investigated. Simultaneously the use of a previously described PCR diagnostic system was assessed for its ability to identify EAggEC infection. E. coli strains isolated during a 1-year case-control study from faecal samples of 388 children aged <5 years, with or without diarrhoea, were examined for EAggEC by HeLa cell adherence assay in parallel with a PCR assay with primers generated from an EAggEC DNA probe. A blind comparison was made between the two methods to determine their diagnostic potential. E. coli isolates that adhered to HeLa cells in an aggregative pattern were the sole isolates significantly more often in 254 cases (9%) than in 134 control (2%) children. Age stratification showed that EAggEC were isolated more frequently from children aged <36 months. The EAggEC isolates belonged to several O serogroups and showed multiple drug resistance. Both methods were positive for 26 samples, nine samples were positive by PCR alone and seven samples were positive by culture alone, thus indicating a 78% sensitivity and 97% specificity for the PCR assay. EAggEC is an important aetiological agent of acute diarrhoea among infants in and around Calcutta, and the PCR diagnostic system may be useful to identify such infection in epidemiological studies.


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
S. Kahali, B. Sarkar, K. Rajendran, J. Khanam, S. Yamasaki, R. K. Nandy, S. K. Bhattacharya, and T. Ramamurthy
Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India
J. Clin. Microbiol., September 1, 2004; 42(9): 4111 - 4120.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
O.-A. Ratchtrachenchai, S. Subpasu, H. Hayashi, and W. Ba-Thein
Prevalence of childhood diarrhoea-associated Escherichia coli in Thailand
J. Med. Microbiol., March 1, 2004; 53(3): 237 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
E. PRESTERL, R. H. ZWICK, S. REICHMANN, A. AICHELBURG, S. WINKLER, P. G. KREMSNER, and W. GRANINGER
FREQUENCY AND VIRULENCE PROPERTIES OF DIARRHEAGENIC ESCHERICHIA COLI IN CHILDREN WITH DIARRHEA IN GABON
Am J Trop Med Hyg, October 1, 2003; 69(4): 406 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
W. L. Pabst, M. Altwegg, C. Kind, S. Mirjanic, D. Hardegger, and D. Nadal
Prevalence of Enteroaggregative Escherichia coli among Children with and without Diarrhea in Switzerland
J. Clin. Microbiol., June 1, 2003; 41(6): 2289 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
S. Dutta, A. Deb, U.K. Chattopadhyay, and T. Tsukamoto

J. Med. Microbiol., August 1, 2000; 49(8): 765 - 767.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1999 Society for General Microbiology.