J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Singh, M.
Right arrow Articles by Ganguly, N. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Singh, M.
Right arrow Articles by Ganguly, N. K.
Agricola
Right arrow Articles by Singh, M.
Right arrow Articles by Ganguly, N. K.

The Journal of Medical Microbiology, Vol 48, Issue 1 79-88, Copyright © 1999 by Society for General Microbiology

JOURNAL ARTICLE

Induction of systemic and mucosal immune response in mice immunised with porins of Salmonella typhi

M. Singh, H. Vohra, L. Kumar and N. K. Ganguly
Department of Paediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Porins, purified from Salmonella typhi strain 0901 provided 90% protection to BALB/c mice against a lethal dose (300 x LD50) of S. typhi Ty2 when given intraperitoneally. To measure the porin-induced cellular immune responses, macrophages and lymphocytes were isolated from spleen and lamina propria (LP) of porin immunised-challenge mice and of infected and control mice; T-cell phenotypes, lymphocyte proliferation and cytokine production were studied. The secretory IgA (sIgA) antibody level in the intestinal fluid was also measured to study mucosal immune response. After immunisation, the splenic lymphocytes exhibited a significant increase in total T-cell count and CD4+/CD8+ ratio, while the LP lymphocytes (LPL) exhibited an increase in CD4+/CD8+ ratio only. They also exhibited a significant increase in porin-specific proliferative response and cytokine levels (IL-1, IL-2, IFN-gamma and IL-4). After immunisation, sIgA antibody was also found to be increased. These results suggest that porins given intraperitoneally induce cellular and humoral immune responses both at systemic and mucosal levels.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
S. Sood, P. Rishi, H. Vohra, S. Sharma, and N. K Ganguly
Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels
J. Med. Microbiol., September 1, 2005; 54(9): 815 - 821.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
V. Chanana, R. Sehgal, and P. Rishi
Salmonella typhi iron-regulated outer-membrane proteins cause oedema and hyperalgesia during inflammation induced in a rat model
J. Med. Microbiol., April 1, 2005; 54(4): 421 - 423.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Fernandez-Mora, J. L. Puente, and E. Calva
OmpR and LeuO Positively Regulate the Salmonella enterica Serovar Typhi ompS2 Porin Gene
J. Bacteriol., May 15, 2004; 186(10): 2909 - 2920.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Q. Zhang, J. C. Meitzler, S. Huang, and T. Morishita
Sequence Polymorphism, Predicted Secondary Structures, and Surface-Exposed Conformational Epitopes of Campylobacter Major Outer Membrane Protein
Infect. Immun., October 1, 2000; 68(10): 5679 - 5689.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1999 Society for General Microbiology.