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The Journal of Medical Microbiology, Vol 47, Issue 10 933-936, Copyright © 1998 by Society for General Microbiology

JOURNAL ARTICLE

Development of a rapid method for quantitative evaluation of Mycobacterium tuberculosis growth based on competitive polymerase chain reaction

M. Ligozzi, E. Pelosi and R. Fontana
Institute of Microbiology, University of Verona, Italy.

A competitive polymerase chain reaction (cPCR) assay for the quantitative evaluation of Mycobacterium tuberculosis growth was developed based on co-amplification of genomic DNA and a modified DNA fragment derived from a well-conserved region of the 16S rRNA gene. There was a good correlation between the number of DNA copies in the sample, indicated by competitive PCR, and the number of colony forming units determined by conventional culture methods.




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