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J Med Microbiol 46 (1997), 665-668; DOI: 10.1099/00222615-46-8-665
© 1997 Society for General Microbiology
ISSN 0022-2615
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Detection of Pneumocystis carinii DNA by PCR amplification in various rat organs in experimental pneumocystosis

M. RABODONIRINA, R. WILMOTTE*, E. DANNAOUI, F. PERSAT, G. BAYLE and M. MOJON{dagger}

Departement de Parasitologie et de Mycologie, Université Claude-Bernard, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France

*Génétique Moléculaire Humaine, CNRS URA 1171, Institut Pasteur de Lyon, Avenue Tony Garnier, 69365 Lyon Cedex 07, France

Corresponding author: Dr M. Rabodonirina.

Received September 13, 1996 Revision received December 29, 1996.
Accepted December 29, 1996

Pneumocystosis is usually a disease of the lungs, but the number of cases of extrapulmonary pneumocystosis has greatly increased during the AIDS epidemic. Much remains unknown about the frequency and mechanisms of dissemination. In the present study, a systematic search for Pneumocystis carinii by PCR with primers specific for mitochondrial rRNA was performed in the lung, liver, spleen and kidney of 12 immunosuppressed rats and two immunocompetent rats. The amplified products were analysed by Southern hybridisation with a digoxigenin-11-dUTP labeled probe. P. carinii DNA was found in lungs in all 14 rats and in at least one organ other than lung in 11 immunosuppressed rats and the two control rats. We suggest that extrapulmonary dissemination may not be an exceptional phenomenon in the course of pneumocystosis, but rather part of the natural evolution of the disease.

{dagger} Deceased: this paper is dedicated to her memory.






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Copyright © 1997 Society for General Microbiology.