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J Med Microbiol 46 (1997), 597-601; DOI: 10.1099/00222615-46-7-597
© 1997 Society for General Microbiology
ISSN 0022-2615
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Evaluation of different techniques in the diagnosis of Toxoplasma encephalitis

J. C. RODRÍGUEZ, M. M. MARTÍNEZ*, A. R. MARTÍNEZ* and G. ROYO

Laboratory of Microbiology, General University Hospital of Elche, University of Alicante

*Department of Parasitology, Faculty of Pharmacy, Complutense University, Madrid, Spain

Corresponding author: Dr J. C. Rodriguez.

Received July 2, 1996 Revision received November 22, 1996.
Accepted November 22, 1996

This study evaluated the detection of antibodies, circulating antigens and parasite DNA by polymerase chain reaction (PCR) in the diagnosis of toxoplasma encephalitis. The detection of antibody classes and IgG avidity were not useful diagnostically. The detection of circulating antigens by the ELISA system described was not sufficiently sensitive. The detection of DNA by PCR was the most useful test especially in untreated patients, with a sensitivity of 62% overall, 81% in untreated patients and only 20% in treated patients. The use of non-isotopic probes makes the use of this technique feasible in routine diagnostic parasitology laboratories.




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