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Pulsed-Field Gel Electrophoresis Genomic Fingerprinting of Hospital Escherichia Coli Bacteraemia Isolates

¹Department of Pediatrics, Division of Infectious Diseases

^*Clinical Microbiology, University of Michigan, Ann Arbor, Michigan 48109-0244, USA

²Corresponding author: Dr R. A. Blackwood.

Received September 23, 1996
Accepted October 30, 1996

Pulsed-field gel electrophoresis (PFGE), because of the increased sensitivity it affords over other methods of bacterial genotyping, represents a potentially powerful tool for the characterisation of isolates from hospital infections. Genomic fingerprinting by PFGE was applied to all clinical isolates of Escherichia coli obtained from blood during a 6month period (78 isolates, 58 patients) at the University of Michigan Medical Center. The rare-restriction patterns of these isolates, in contrast to those of isolates from the E. coli reference collection (ECOR), were not randomly distributed through the E. coli species. Four related clusters, which represented c. 21% of the blood isolates, were identified. Two of these genotypic clusters were also clustered temporally, their members all being isolated within the same 2-week period, while the other two clusters spanned the study period. These observations indicate in-hospital endemic vectors or the occurrence of specialised E. coli lineages that are capable of invading the bloodstream and exploiting in-hospital vectors, or both.

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