J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sharma, M.
Right arrow Articles by Sehgal, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharma, M.
Right arrow Articles by Sehgal, S.
Agricola
Right arrow Articles by Sharma, M.
Right arrow Articles by Sehgal, S.

The Journal of Medical Microbiology, Vol 46, Issue 12 1039-1042, Copyright © 1997 by Society for General Microbiology

JOURNAL ARTICLE

Low sensitivity of counter-current immuno-electrophoresis for serodiagnosis of typhoid fever

M. Sharma, U. Datta, P. Roy, S. Verma and S. Sehgal
Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Counter-current immuno-electrophoresis was evaluated as a diagnostic test for the serodiagnosis of typhoid fever with somatic (O), flagellar (H) and capsular polysaccharide (Vi) antigens of Salmonella typhi on the sera of patients who were blood culture positive (confirmed typhoid cases) or had high Widal agglutination titres, > or = 320, (presumptive typhoid cases). Of the 37 sera from confirmed cases, 30% showed positivity with O antigen, 24% with H antigens and 51% with Vi antigen. In patients with a presumptive diagnosis, 45% were positive for O antibody, 27% for flagellar antibody and 52% for Vi antibody. When all three antigens were combined the reactivity to any of the antigens was found to be 59% in confirmed typhoid cases, 79% in presumptive typhoid cases and 93% in patients who were simultaneously positive by blood culture and Widal agglutination. However, none of the sera from 45 controls gave a positive precipitation reaction with any of the antigens. It is concluded that counter-current immuno-electrophoresis is a rapid test with low sensitivity and high specificity with Vi antigen, a panel of antigens being most effective, and is, therefore, recommended for rapid diagnosis of typhoid fever.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
F. C. H. Tam, T. K. W. Ling, K. T. Wong, D. T. M. Leung, R. C. Y. Chan, and P. L. Lim
The TUBEX test detects not only typhoid-specific antibodies but also soluble antigens and whole bacteria
J. Med. Microbiol., March 1, 2008; 57(3): 316 - 323.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1997 Society for General Microbiology.