The Journal of Medical Microbiology, Vol 45, Issue 6 413-418, Copyright © 1996 by Society for General Microbiology
Genomic lineage of Salmonella enterica serotype Gallinarum
J. E. Olsen, M. N. Skov, J. P. Christensen and M. Bisgaard
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Forty-eight strains of Salmonella enterica serotype Gallinarum of biotypes
Gallinarum and Pullorum were characterised by three chromosomally based
typing methods. The patterns obtained were compared with those of strains
of eight other serotypes of Salmonella of O serogroup D. The same PvuII and
PstI IS200 patterns were commonly observed among strains of both biotypes
and the three SmaI ribotypes of serotype Gallinarum strains differed in
only one or two bands, supporting the view that members of these two
biotypes are closely related. The same IS200 patterns were also commonly
observed among strains of serotype Enteritidis, indicating its evolutionary
relationship with serotype Gallinarum. NotI pulsed-field gel
electrophoresis (PFGE) patterns divided strains into 24 types. Based on a
similarity analysis, two clusters were formed. One contained the majority
of biotype Gallinarum strains and two atypical strains of Pullorum; the
other contained strains of biotype Pullorum and an otherwise typical strain
of biotype Gallinarum. Two atypical strains of biotype Pullorum remained
unclustered by PFGE analysis. The grouping of strains differed according to
the typing method used, but the majority of strains within each of the
biotypes Gallinarum and Pullorum were very similar by the chromosomal
markers analysed.
