J Med Microbiol NEW Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Freeman, R.
Right arrow Articles by Burdess, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freeman, R.
Right arrow Articles by Burdess, D.
Agricola
Right arrow Articles by Freeman, R.
Right arrow Articles by Burdess, D.

The Journal of Medical Microbiology, Vol 45, Issue 5 349-352, Copyright © 1996 by Society for General Microbiology

JOURNAL ARTICLE

Heterogeneity within apparently pure cultures of Escherichia coli freshly isolated from significant bacteriuria

R. Freeman, P. R. Sisson and D. Burdess
Regional Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne.

Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, the set of 10 single-colony isolates proved indistinguishable by PyMS. However, for four urines there was clear heterogeneity. For two urines, one of the isolates was distinct, and the remaining nine indistinguishable. One urine yielded seven indistinguishable isolates, and three further individually distinct isolates. One urine yielded two clusters of indistinguishable isolates, one comprising seven and the other three isolates. Thus, significant heterogeneity (as measured by PyMS) occurs in apparently pure cultures of E. coli from urinary tract infection. The nature of this heterogeneity remains to be established, as does the significance, if any, of this phenomenon in urinary tract infection.


This article has been cited by other articles:


Home page
 J. Clin. Pathol.Home page
J C Graham and A Galloway
ACP Best Practice No 167: The laboratory diagnosis of urinary tract infection
J. Clin. Pathol., December 1, 2001; 54(12): 911 - 919.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1996 Society for General Microbiology.