J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mastrantonio, P.
Right arrow Articles by Giuliano, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mastrantonio, P.
Right arrow Articles by Giuliano, M.
Agricola
Right arrow Articles by Mastrantonio, P.
Right arrow Articles by Giuliano, M.

The Journal of Medical Microbiology, Vol 44, Issue 4 261-266, Copyright © 1996 by Society for General Microbiology

JOURNAL ARTICLE

Polymerase chain reaction for the detection of Bordetella pertussis in clinical nasopharyngeal aspirates

P. Mastrantonio, P. Stefanelli and M. Giuliano
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy.

A PCR procedure for the detection of Bordetella pertussis in nasopharyngeal aspirates (NPAs) was developed with primers derived from the pertussis toxin promoter region. The amplification resulted in a 191-bp PCR product specific for B. pertussis. A total of 681 NPAs collected from children with cough lasting >7 days was evaluated by PCR and culture; 104 aspirates were positive by PCR and 93 by culture. Sixteen cases were positive only by PCR and five culture positive aspirates were negative by PCR. An internal control was included in the assay to monitor the performance of the PCR and to identify possible inhibitory components in clinical samples. The PCR method was more efficient than culture in detecting B. pertussis in samples collected late in the disease, in antibiotic-treated children and in patients with mild disease.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
D. M. Dragsted, B. Dohn, J. Madsen, and J. S. Jensen
Comparison of culture and PCR for detection of Bordetella pertussis and Bordetella parapertussis under routine laboratory conditions
J. Med. Microbiol., August 1, 2004; 53(8): 749 - 754.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
N. K. Fry, O. Tzivra, Y. T. Li, A. McNiff, N. Doshi, P. A.C. Maple, N. S. Crowcroft, E. Miller, R. C. George, and T. G. Harrison
Laboratory diagnosis of pertussis infections: the role of PCR and serology
J. Med. Microbiol., June 1, 2004; 53(6): 519 - 525.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
L. M. Sloan, M. K. Hopkins, P. S. Mitchell, E. A. Vetter, J. E. Rosenblatt, W. S. Harmsen, F. R. Cockerill, and R. Patel
Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens
J. Clin. Microbiol., January 1, 2002; 40(1): 96 - 100.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child.Home page
H. Clements, T. Stephenson, V. Gabriel, T. Harrison, M. Millar, A. Smyth, W. Tong, and C. J Linton
Rationalised prescribing for community acquired pneumonia: a closed loop audit
Arch. Dis. Child., October 1, 2000; 83(4): 320 - 324.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
U. Heininger, G. Schmidt-Schläpfer, J. D. Cherry, and K. Stehr
Clinical Validation of a Polymerase Chain Reaction Assay for the Diagnosis of Pertussis by Comparison With Serology, Culture, and Symptoms During a Large Pertussis Vaccine Efficacy Trial
Pediatrics, March 1, 2000; 105(3): 31e - 31.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1996 Society for General Microbiology.