J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grundmann, H.
Right arrow Articles by Daschner, F. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grundmann, H.
Right arrow Articles by Daschner, F. D.
Agricola
Right arrow Articles by Grundmann, H.
Right arrow Articles by Daschner, F. D.

The Journal of Medical Microbiology, Vol 43, Issue 6 446-451, Copyright © 1995 by Society for General Microbiology

JOURNAL ARTICLE

Automated laser fluorescence analysis of randomly amplified polymorphic DNA: a rapid method for investigating nosocomial transmission of Acinetobacter baumannii

H. Grundmann, C. Schneider, H. V. Tichy, R. Simon, I. Klare, D. Hartung and F. D. Daschner
Institut fur Umweltmedizin und Krankenhaushygiene, Universitatsklinik Freiburg, Germany.

A rapid method for genotyping Acinetobacter baumannii based on PCR-fingerprinting with fluorescent primers was evaluated. Automated laser fluorescence analysis (ALFA) enabled on-line generation of high resolution DNA-fingerprints during polyacrylamide gel electrophoresis of randomly amplified polymorphic DNA (RAPD) products. The results were in concordance with macro-restriction fragment patterns produced by pulse-field gel electrophoresis (PFGE) of ApaI digests of chromosomal DNA. RAPD-ALFA was able to identify homologous strains suggestive of horizontal transmission in < 8 h after colonies were obtained on solid media, whereas PFGE analysis took c. 90 h. Speed and digitised data format renders RAPD-ALFA attractive for routine in-house epidemiological screening of isolates from intensive care and other hospital units.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
R. P. SPENCE, K. J. TOWNER, C. J. HENWOOD, D. JAMES, N. WOODFORD, and D. M. LIVERMORE
Population structure and antibiotic resistance of Acinetobacter DNA group 2 and 13TU isolates from hospitals in the UK
J. Med. Microbiol., December 1, 2002; 51(12): 1107 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
D. Jonas, H.-G. W. Meyer, P. Matthes, D. Hartung, B. Jahn, F. D. Daschner, and B. Jansen
Comparative Evaluation of Three Different Genotyping Methods for Investigation of Nosocomial Outbreaks of Legionnaires' Disease in Hospitals
J. Clin. Microbiol., June 1, 2000; 38(6): 2284 - 2291.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1995 Society for General Microbiology.