J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clark, C. G.
Right arrow Articles by Johnson, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clark, C. G.
Right arrow Articles by Johnson, R. P.
Agricola
Right arrow Articles by Clark, C. G.
Right arrow Articles by Johnson, R. P.

The Journal of Medical Microbiology, Vol 43, Issue 4 262-269, Copyright © 1995 by Society for General Microbiology

JOURNAL ARTICLE

Further characterisation of a monoclonal antibody reactive with Escherichia coli O157:H7

C. G. Clark, S. Johnson and R. P. Johnson
Agriculture and Agri-Food Canada, Health of Animals Laboratory, Guelph, Ontario.

Monoclonal antibody (MAb) 4E8C12 has been previously reported to recognise low mol. wt proteins from enterohaemorrhagic Escherichia coli (EHEC) serotypes O157:H7 and O26:H11. Crude lipopolysaccharide (LPS) preparations from proteinase K-digested bacterial suspensions reacted in Western blots with MAb 4E8C12, as did highly purified LPS from O157:H7 strains. The material recognised by this antibody was, therefore, LPS. The LPS epitope was identified by a whole-cell ELISA in several EHEC, verotoxin producing E. coli (VTEC) and verotoxin-negative strains in addition to E. coli serotypes O157:H7 and O26:H11. Acriflavine and bile salts enhanced the production or availability of the epitope at the cell surface and in culture supernates. These data indicate that the presence of the epitope did not correlate with the virulence of these organisms.


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
W.-L. Tsai, C. E. Miller, and E. R. Richter
Determination of the Sensitivity of a Rapid Escherichia coli O157:H7 Assay for Testing 375-Gram Composite Samples
Appl. Envir. Microbiol., September 1, 2000; 66(9): 4149 - 4151.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1995 Society for General Microbiology.