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The Journal of Medical Microbiology, Vol 39, Issue 6 473-475, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Serum hepatitis B virus DNA detection with S- and C-region-directed probes

F. Garcia, E. Quiros, M. C. Bernal, B. De Luis, A. Leyva, G. Piedrola and M. C. Maroto
Department of Microbiology, Faculty of Medicine, University of Granada, Spain.

Developments in molecular biology have offered a wide range of nucleic acid probes to detect the genome of hepatitis B virus (HBV). We have tested the ability of two enzyme-linked (alkaline phosphatase) probes to detect HBV-DNA. These hybridise with the S and C regions of the genome of HBV and are used to determine the clinical significance of detecting the two regions. A total of 66 serum samples from patients at different stages of HBV infection was examined. HBV-DNA was detected with at least one of the probes in 17 (85%) patients with HBeAg-positive chronic hepatitis, five (26.3%) with anti-HBe-positive chronic hepatitis and six (66.6%) with acute hepatitis. Although both probes were able to detect as little as 10 pg/ml (2.86 x 10(6) g.E./ml) of a full length HBV-DNA standard, the C-region-directed probe did not react in one patient with acute hepatitis, two with HBeAg-positive and three with anti-HBe-positive chronic hepatitis. When C-region-directed probes are used for diagnostic purposes, results should always be accompanied by hybridisation with probes directed against other regions showing less variability (e.g. S region).


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Quantitative Detection of Hepatitis B Virus by Transcription-Mediated Amplification and Hybridization Protection Assay
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[Abstract] [Full Text]


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