J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kellens, J. T.
Right arrow Articles by Stobberingh, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kellens, J. T.
Right arrow Articles by Stobberingh, E. E.
Agricola
Right arrow Articles by Kellens, J. T.
Right arrow Articles by Stobberingh, E. E.

The Journal of Medical Microbiology, Vol 39, Issue 6 440-445, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

The agglutination of beta-haemolytic streptococci by lectins

J. T. Kellens, J. A. Jacobs, W. J. Peumans and E. E. Stobberingh
Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands.

The ability of 25 lectins, isolated from different plants and fungi, to agglutinate 95 clinical isolates of beta-haemolytic streptococci was examined. Cell suspensions were untreated, trypsin-treated or boiled at pH 2.0. None of the 95 untreated cell suspensions gave a visible reaction with any of the lectins. When the cells were trypsinised, 42 strains were agglutinated with one or more lectin and after boiling at pH 2, all the strains were agglutinated. After treatment with trypsin, 20 different agglutination patterns were observed, and after boiling, 19 patterns, four of which were similar. A correlation was found between Lancefield group C and some of these patterns. Some lectins reacted specifically with group C streptococci; DBA and WFA, both specific for D-GalNAc, DSA, a GlcNAc-specific lectin, and RPA, which showed a complex specificity, reacted only with group C strains. Furthermore, the lectin of Maackia amurensis reacted with 50% of group B streptococci only. Agglutination assays with lectins were reproducible, easy to perform, relatively inexpensive and, therefore, applicable to studies of cell-wall structure and epidemiology of beta-haemolytic streptococci.


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
G. Robitaille, S. Moineau, D. St-Gelais, C. Vadeboncoeur, and M. Britten
Detection and quantification of capsular exopolysaccharides from Streptococcus thermophilus using lectin probes.
J Dairy Sci, November 1, 2006; 89(11): 4156 - 4162.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
I. H. Ekin and K. Gurturk
Characterization of bovine and human group B streptococci isolated in Turkey.
J. Med. Microbiol., May 1, 2006; 55(Pt 5): 517 - 521.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1993 Society for General Microbiology.