J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramamurthy, T.
Right arrow Articles by et, al.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramamurthy, T.
Right arrow Articles by et, al.
Agricola
Right arrow Articles by Ramamurthy, T.
Right arrow Articles by et, al.

The Journal of Medical Microbiology, Vol 39, Issue 4 310-317, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Virulence patterns of Vibrio cholerae non-O1 strains isolated from hospitalised patients with acute diarrhoea in Calcutta, India

T. Ramamurthy, P. K. Bag, A. Pal, S. K. Bhattacharya, M. K. Bhattacharya, T. Shimada, T. Takeda, T. Karasawa, H. Kurazono, Y. Takeda and al. et
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.

A collection of 28 strains of Vibrio cholerae non-O1 isolated during a 3-year period (1989-1991) from hospitalised patients with acute diarrhoea in Calcutta, India, were examined with regard to virulence-associated factors. Of the 28 isolates (each representing a case), 18 were isolated as the sole infecting agent; the remaining 10 were recovered as co-cultures from cases infected with V. cholerae O1. Of the strains isolated in this study, 82% could be serotyped, with serovars O5 (32.1%), O11 and O34 (14.3% each) predominant. Serovars O7, O14, O34, O39 and O97 were associated exclusively with sole infections. Two strains of V. cholerae non-O1 produced anti-cholera toxin IgG-absorbable cholera toxin (CT). Both CT-producing V. cholerae non-O1 strains hybridised with the DNA probe specific for the zonula occludens toxin (ZOT) but none of the remaining 26 strains hybridised with the ZOT probe. The majority of the strains were cytotoxic for CHO, HeLa and Vero cells, with end-point titres of 4-512. Fewer strains produced a cytotonic effect, with end-point titres of 2-16. Of the 28 strains of V. cholerae non-O1 examined, 75%, 75%, 25% and 14.3% produced haemolysin that was active against erythrocytes of rabbit, sheep (Eltor haemolysin), chicken and man, respectively. Strains that produced a haemolysin active against both rabbit and sheep erythrocytes were dominant (35.7%). Ten (35.7%) of the 28 strains examined showed cell-associated haemagglutinating activity on human blood. Of the 10 strains, nine were isolated as sole pathogen and only one strain was associated with mixed infection.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
R. Chakraborty, S. Chakraborty, K. De, S. Sinha, A. K Mukhopadhyay, J. Khanam, T. Ramamurthy, Y. Takeda, S. K Bhattacharya, and G B. Nair
Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea
J. Med. Microbiol., August 1, 2005; 54(8): 707 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. M. Faruque, N. Chowdhury, M. Kamruzzaman, M. Dziejman, M. H. Rahman, D. A. Sack, G. B. Nair, and J. J. Mekalanos
Genetic diversity and virulence potential of environmental Vibrio cholerae population in a cholera-endemic area
PNAS, February 17, 2004; 101(7): 2123 - 2128.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. K. Mukhopadhyay, S. Chakraborty, Y. Takeda, G. B. Nair, and D. E. Berg
Characterization of VPI Pathogenicity Island and CTX{phi} Prophage in Environmental Strains of Vibrio cholerae
J. Bacteriol., August 15, 2001; 183(16): 4737 - 4746.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
I. N. G. Rivera, J. Chun, A. Huq, R. B. Sack, and R. R. Colwell
Genotypes Associated with Virulence in Environmental Isolates of Vibrio cholerae
Appl. Envir. Microbiol., June 1, 2001; 67(6): 2421 - 2429.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
P. Figueroa-Arredondo, J. E. Heuser, N. S. Akopyants, J. H. Morisaki, S. Giono-Cerezo, F. Enriquez-Rincon, and D. E. Berg
Cell Vacuolation Caused by Vibrio cholerae Hemolysin
Infect. Immun., March 1, 2001; 69(3): 1613 - 1624.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
S. Chakraborty, A. K. Mukhopadhyay, R. K. Bhadra, A. N. Ghosh, R. Mitra, T. Shimada, S. Yamasaki, S. M. Faruque, Y. Takeda, R. R. Colwell, et al.
Virulence Genes in Environmental Strains of Vibrio cholerae
Appl. Envir. Microbiol., September 1, 2000; 66(9): 4022 - 4028.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
R. Mitra, P. Figueroa, A. K. Mukhopadhyay, T. Shimada, Y. Takeda, D. E. Berg, and G. B. Nair
Cell Vacuolation, a Manifestation of the El Tor Hemolysin of Vibrio cholerae
Infect. Immun., April 1, 2000; 68(4): 1928 - 1933.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. Zhang, J. Takahashi, T. Seno, Y. Tani, and T. Honda
Analysis of Receptor for Vibrio cholerae El Tor Hemolysin with a Monoclonal Antibody That Recognizes Glycophorin B of Human Erythrocyte Membrane
Infect. Immun., October 1, 1999; 67(10): 5332 - 5337.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1993 Society for General Microbiology.