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The Journal of Medical Microbiology, Vol 39, Issue 4 262-267, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

The production of porphyrins from delta-aminolaevulinic acid by Haemophilus parainfluenzae

P. Luppa, K. Jacob and W. Ehret
Institute of Clinical Chemistry, University Hospital Grosshadern, University of Munich, Germany.

Porphyrin production by the haemin-independent Haemophilus parainfluenzae in the diagnostic porphyrin test, which determines the X-factor requirement in Haemophilus spp., was analysed quantitatively by applying modern high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9-8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.




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