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The Journal of Medical Microbiology, Vol 38, Issue 5 366-370, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Subtyping of Neisseria gonorrhoeae auxotype-serovar groups by pulsed-field gel electrophoresis

C. L. Poh and Q. C. Lau
Department of Microbiology, Faculty of Medicine, National University of Singapore.

Genomic DNA from 48 Neisseria gonorrhoeae isolates was digested with low-frequency cleavage (LFC) endonucleases (SpeI and NheI) and analysed by contour-clamped homogeneous electric fields electrophoresis (CHEF). The restriction patterns generated were reproducible, stable, easy to read and offer a more practical approach than restriction endonuclease analysis (REA) with high-frequency cleavage (HFC) endonucleases. Strains sharing common auxotypes and serovars could be differentiated and correlation with auxotype/serovar (A/S) classes was demonstrated for some, but not all strains. The strains were distributed into 18 A/S classes and 38 SpeI and 40 NheI restriction patterns. This greater discriminatory power of CHEF REA should allow subdivision of strains within common A/S classes.


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