J Med Microbiol NEW Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wastling, J. M.
Right arrow Articles by Buxton, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wastling, J. M.
Right arrow Articles by Buxton, D.
Agricola
Right arrow Articles by Wastling, J. M.
Right arrow Articles by Buxton, D.

The Journal of Medical Microbiology, Vol 38, Issue 5 360-365, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Comparison of two gene amplification methods for the detection of Toxoplasma gondii in experimentally infected sheep

J. M. Wastling, S. Nicoll and D. Buxton
Moredun Research Institute, Edinburgh.

Efferent lymph and peripheral blood collected from sheep experimentally infected with Toxoplasma gondii strain S48 were analysed for parasite DNA by amplification of the B1 and P30 T. gondii genes by the polymerase chain reaction (PCR). The relative sensitivity of these two gene amplification methods was assessed and compared with parasite detection by mouse injection (MI). B1 PCR was consistently more sensitive than P30 PCR and the results agreed closely with those from MI. By contrast, P30 PCR gave more than twice as many false negatives results than B1 PCR. The few apparent false positive results given by either PCR method were probably due to the inability of MI to detect non-viable parasites. All specimens collected before infection with T. gondii gave negative results by PCR and MI. Parasite DNA was detected by both B1 and P30 PCR in the lymph node of a sheep 12 days after infection but not in other tissues. The results permit a direct comparison between T. gondii detection by P30 and B1 PCR. Moreover, they further confirm the value of PCR detection of toxoplasma as a sensitive, specific and reliable diagnostic and research tool.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
J. HAFID, P. FLORI, H. RABERIN, and R. TRAN MANH SUNG
Comparison of PCR, capture ELISA and immunoblotting for detection of Toxoplasma gondii in infected mice
J. Med. Microbiol., December 1, 2001; 50(12): 1100 - 1104.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Haumont, L. Delhaye, L. Garcia, M. Jurado, P. Mazzu, V. Daminet, V. Verlant, A. Bollen, R. Biemans, and A. Jacquet
Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model
Infect. Immun., September 1, 2000; 68(9): 4948 - 4953.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1993 Society for General Microbiology.