J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Finkelstein, R.
Right arrow Articles by Sobel, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Finkelstein, R.
Right arrow Articles by Sobel, J. D.
Agricola
Right arrow Articles by Finkelstein, R.
Right arrow Articles by Sobel, J. D.

The Journal of Medical Microbiology, Vol 38, Issue 3 183-186, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Diagnostic efficacy of a DNA probe in pneumonia caused by Legionella species

R. Finkelstein, P. Brown, W. A. Palutke, B. B. Wentworth, J. G. Geiger, G. D. Bostic and J. D. Sobel
Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan.

A commercial DNA probe kit (Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires' disease in 167 patients with pneumonia. The test was performed on freshly obtained clinical respiratory tract samples. Cultures and direct immunofluorescence antibody (DFA) staining of the samples and serological tests were performed simultaneously for all patients. The probe assay result was positive in six patients; five of them had other laboratory evidence of disease (positive cultures or positive serological results or both). Depending on the diagnostic criteria, the probe test had a sensitivity of 31-67%, a specificity of 99% and positive predictive values of 67-83%. The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. The results indicate that the examination of respiratory tract secretions by the Gen-probe kit is a suitable screening test for the diagnosis of Legionnaires' disease.


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
B. S. Fields, R. F. Benson, and R. E. Besser
Legionella and Legionnaires' Disease: 25 Years of Investigation
Clin. Microbiol. Rev., July 1, 2002; 15(3): 506 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. T. Hayden, J. R. Uhl, X. Qian, M. K. Hopkins, M. C. Aubry, A. H. Limper, R. V. Lloyd, and F. R. Cockerill
Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture
J. Clin. Microbiol., July 1, 2001; 39(7): 2618 - 2626.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1993 Society for General Microbiology.