J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meng, X. Q.
Right arrow Articles by Nakamura, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meng, X. Q.
Right arrow Articles by Nakamura, S.
Agricola
Right arrow Articles by Meng, X. Q.
Right arrow Articles by Nakamura, S.

The Journal of Medical Microbiology, Vol 38, Issue 1 69-73, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Purification and characterisation of intracellular toxin A of Clostridium difficile

X. Q. Meng, S. Kamiya, K. Yamakawa, H. Ogura and S. Nakamura
Department of Bacteriology, School of Medicine, Kanazawa University, Japan.

After sonic disintegration of Clostridium difficile cells, intracellular toxin A was purified to homogeneity by thyroglobulin affinity chromatography (TGAC) followed by anion-exchange (Mono Q) by fast protein liquid chromatography (FPLC). High haemagglutinating (HA) activity was detected in TGAC-unbound fractions (2(9)/50 microliters), but not in TGAC thermal eluates (2(0)/50 microliters). The low HA titre of the thermal eluates was markedly increased to 2(5)/50 microliters after dialysis against 0.02 M Tris-HCl (pH 7.5). A disparity in the position of the peaks containing cytotoxic and HA activity was observed in the first Mono Q-FPLC step. Intracellular toxin A without HA activity was obtained by a second Mono Q-FPLC step. The M(r) of the intracellular toxin A was estimated by polyacrylamide gel electrophoresis (PAGE) to be 580 kDa under non-denaturing conditions. The minimum doses of the toxin causing cytotoxicity, mouse lethality and enterotoxicity were 0.83 ng, 8.7 ng and 5 micrograms, respectively.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y. Ishida, T. Maegawa, T. Kondo, A. Kimura, Y. Iwakura, S. Nakamura, and N. Mukaida
Essential Involvement of IFN-{gamma} in Clostridium difficile Toxin A-Induced Enteritis
J. Immunol., March 1, 2004; 172(5): 3018 - 3025.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1993 Society for General Microbiology.