J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Owen, R. J.
Right arrow Articles by Linton, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Owen, R. J.
Right arrow Articles by Linton, D.
Agricola
Right arrow Articles by Owen, R. J.
Right arrow Articles by Linton, D.

The Journal of Medical Microbiology, Vol 38, Issue 1 6-12, Copyright © 1993 by Society for General Microbiology

JOURNAL ARTICLE

Biotype and molecular fingerprints of metronidazole-resistant strains of Helicobacter pylori from antral gastric mucosa

R. J. Owen, G. D. Bell, M. Desai, M. Moreno, P. W. Gant, P. H. Jones and D. Linton
National Collection of Type Cultures, Central Public Health Laboratory, London.

Biotypes, ribosomal RNA gene restriction patterns (ribopatterns), whole-cell protein patterns and plasmid profiles of paired Helicobacter pylori isolates from 17 patients were examined. Each pair comprised a pre- and a post-treatment isolate; nine of the 17 post-treatment isolates were obtained after treatment with tripotassium dicitrate bismuthate (De-Nol) and metronidazole. All strains of H. pylori had identical biotypes, but exhibited diversity between pairs in their molecular fingerprints. Each of the 17 strain pairs had unique ribopatterns; the pre- and post-treatment isolates in most pairs (16 of 17) were similar or identical, irrespective of metronidazole susceptibility. DNA subtype variants were detected in three patient sets. Although nine post-treatment isolates had acquired resistance to metronidazole, most (six of nine) resembled the pre-treatment isolates in their ribopattern, protein and plasmid profiles. No significant correlation was observed between metronidazole resistance and plasmid content in these H. pylori isolates. Emergence of post-treatment metronidazole-resistant isolates of H. pylori was associated only rarely with colonisation by a novel strain or acquisition of a plasmid and, in most patients, probably resulted from spontaneous emergence of resistance in the original infecting strain.


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
C. Burucoa, V. Lhomme, and J. L. Fauchere
Performance Criteria of DNA Fingerprinting Methods for Typing of Helicobacter pylori Isolates: Experimental Results and Meta-Analysis
J. Clin. Microbiol., December 1, 1999; 37(12): 4071 - 4080.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
D. Morrison, N. Woodford, S. P. Barrett, P. Sisson, and B. D. Cookson
DNA Banding Pattern Polymorphism in Vancomycin-Resistant Enterococcus faecium and Criteria for Defining Strains
J. Clin. Microbiol., April 1, 1999; 37(4): 1084 - 1091.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1993 Society for General Microbiology.