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In-vitro activity of peritoneal cells from rats after intra-abdominal infection with Bacteroides fragilis and Escherichia coli

W. R. Verweij, F. Namavar, W. F. Schouten, P. J. Kostense^*, M. Pellenkoft, J. De Graaff and D. M. Maclaren

Vrije Universiteit, School of Medicine, Department of Medical Microbiology, Research Group for Commensal Infections

^*Department of Theory of Medicine, Epidemiology and Biostatistics, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

Received February 27, 1992 Revision received April 20, 1992.
Accepted April 20, 1992

Peritoneal cells from rats infected intraperitoneally with Escherichia coli and Bacteroides fragilis, alone or in combination were examined in vitro. Cells were harvested 6 h after implantation of fibrin clots infected with E. coli or B. fragilis, separately or containing both species, and assayed for their bactericidal capacities, chemiluminescence and production of cidal metabolites. Peritoneal cell populations from rats with implants of any of the infected clots showed similar distribution of different subpopulations. Bactericidal activity of peritoneal cells did not differ with the bacterial species used. Chemiluminescence values of peritoneal cells from rats with mono-infected B. fragilis or mixed-infected implanted clots, after stimulation with either particles or chemical stimuli, were significantly higher than those of rats with mono-infected E. coli or sterile clots. The same tendency was seen with regard to the production of cidal metabolites such as hydrogen peroxide and superoxide anions although no significant differences were found.

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