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The Journal of Medical Microbiology, Vol 37, Issue 6 420-424, Copyright © 1992 by Society for General Microbiology

JOURNAL ARTICLE

Identification of surface-exposed Yersinia pestis proteins by radio-iodination and biotinylation

F. G. Abath, A. M. Almeida and L. C. Ferreira
Laboratorio de Immunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, Brazil.

When whole cells (stationary phase) of Yersinia pestis strain EV76 were radiolabelled with Iodogen and 131I, 16 major and 10 minor surface-exposed outer membrane proteins (OMPs) were identified. Labelling with N-hydroxysuccinimidyl 6-biotinylamino-hexanoate (biotin X-NHS) resulted in a complex protein profile detectable after blotting and developing with peroxidase-conjugated avidin. Y. pestis cell fractionation revealed that biotin X-NHS labelled not only OMPs but also proteins of inner cell compartments. Therefore, radiolabelling was the more reliable technique for identifying the OMPs of Y. pestis.


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