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The Journal of Medical Microbiology, Vol 37, Issue 5 332-334, Copyright © 1992 by Society for General Microbiology

JOURNAL ARTICLE

A biphasic system for primary isolation of mycobacteria compared to solid medium and broth culture

S. E. Hoffner, M. Haile and G. Kallenius
Department of Bacteriology, National Bacteriological Laboratory, Stockholm, Sweden.

A biphasic culture system, the MB Check, was compared with conventional culture on Lowenstein-Jensen egg (LJ) solid medium and with Bactec broth culture for primary isolation of mycobacteria from clinical samples. A total of 104 mycobacterial isolates was detected from 985 samples examined by the three methods. The most sensitive primary isolation was with LJ culture and MB Check; these methods detected 93% and 87% of all positive cultures, respectively. MB Check allowed a somewhat more rapid detection than LJ culture. The presence of atypical mycobacteria was demonstrated most rapidly with the Bactec system.




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