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The Journal of Medical Microbiology, Vol 37, Issue 4 286-290, Copyright © 1992 by Society for General Microbiology

JOURNAL ARTICLE

A simple and rapid method for the detection and identification of mycobacteria using mycobactin

R. Barclay, V. Furst and I. Smith
Department of Microbiology, University of Reading.

A system was developed for the identification of mycobacteria such as Mycobacterium tuberculosis and M. avium, by thin layer chromatography of 55Fe-labelled mycobactin. Approximately 2 x 10(3) mycobacteria were detected within 24 h and little operator time or skill was required. M. avium, M. intracellulare and M. scrofulaceum were found to have lower requirements for iron than other mycobacteria and this may influence their growth in host organisms.




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Copyright © 1992 Society for General Microbiology.