J Med Microbiol NEW Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelson, J. W.
Right arrow Articles by Govan, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, J. W.
Right arrow Articles by Govan, J. R.
Agricola
Right arrow Articles by Nelson, J. W.
Right arrow Articles by Govan, J. R.

The Journal of Medical Microbiology, Vol 36, Issue 5 358-365, Copyright © 1992 by Society for General Microbiology

JOURNAL ARTICLE

Production and characterisation of mouse monoclonal antibodies reactive with the lipopolysaccharide core of Pseudomonas aeruginosa

J. W. Nelson, G. R. Barclay, L. R. Micklem, I. R. Poxton and J. R. Govan
Department of Medical Microbiology, University of Edinburgh Medical School, Scotland.

Monoclonal antibodies (MAbs) to the core antigen region of lipopolysaccharide (LPS) of Pseudomonas aeruginosa were produced from mice immunised with whole cells of heat-killed rough mutants of Pseudomonas aeruginosa expressing partial or complete core LPS. MAbs were screened in an enzyme-linked immunosorbent assay (ELISA) against three different antigen cocktails: S-form LPS from three P. aeruginosa strains, R-form LPS from six P. aeruginosa strains and, as a negative control, R-form LPS from Salmonella typhimurium and Escherichia coli. Selected MAbs were subsequently screened against a range of extracted LPS and whole cells from both reference strains and clinical isolates of P. aeruginosa. The antibodies were also screened in ELISA against whole-cell antigens from other Pseudomonas spp. as well as strains of Haemophilus influenzae, Neisseria subflava and Staphylococcus aureus. Five MAbs reacting with the core component of P. aeruginosa LPS were finally selected. Two of these, MAbs 360.7 and 304.1.4, were particularly reactive in immunoblots against unsubstituted core LPS, including that from O-antigenic serotypes of P. aeruginosa. The MAbs also reacted with some of the other Pseudomonas spp., but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia. Cross-reactivity with whole cells from other bacterial species was minimal or not observed. Reactivity of MAbs with some Staph. aureus strains was observed, and binding to the protein A component was implicated. The reactivity of the MAbs was investigated further by flow cytometry and immunogold electronmicroscopy. The suitability of the MAbs for an immunological assay for detection of P. aeruginosa in respiratory secretions from CF patients is discussed.


This article has been cited by other articles:


Home page
 Innate ImmunityHome page
A. De Soyza, A. Silipo, R. Lanzetta, J. R. Govan, and A. Molinaro
Review: Chemical and biological features of Burkholderia cepacia complex lipopolysaccharides
Innate Immunity, June 1, 2008; 14(3): 127 - 144.
[Abstract] [PDF]

Home page
 ThoraxHome page
P M Farrell and J R W Govan
Pseudomonas serology: confusion, controversy, and challenges
Thorax, August 1, 2006; 61(8): 645 - 647.
[Full Text] [PDF]

Home page
 Innate ImmunityHome page
A. D. Vinion-Dubiel and J. B. Goldberg
Review: Lipopolysaccharide of Burkholderia cepacia complex
Innate Immunity, August 1, 2003; 9(4): 201 - 213.
[Abstract] [PDF]

Home page
 J Med MicrobiolHome page
L. P. MANSFIELD and S.J. FORSYTHE
Demonstration of the Rb1 lipopolysaccharide core structure in Salmonella strains with the monoclonal antibody M105
J. Med. Microbiol., April 1, 2001; 50(4): 339 - 344.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1992 Society for General Microbiology.