The Journal of Medical Microbiology, Vol 36, Issue 3 215-219, Copyright © 1992 by Society for General Microbiology

JOURNAL ARTICLE

Variation of the O antigen of Campylobacter jejuni in vivo

S. D. Mills, B. Kuzniar, B. Shames, L. A. Kurjanczyk and J. L. Penner
Department of Microbiology, University of Toronto, Banting Institute, Canada.

During the course of a clinical study on patients with campylobacteriosis, three consecutive isolates of Campylobacter jejuni from the same patient were sent for O-serotyping. Marked differences in the specificities of the O antigens of the isolates were observed between the first and third isolates when a passive haemagglutination assay system developed for serotyping C. jejuni was used. Differences in specificity were also demonstrated by immunoblots of lipopolysaccharides (LPS) from proteinase K-digested whole cells. The three isolates could not be distinguished either by restriction endonuclease analysis of chromosomal DNA, by gel electrophoresis of whole-cell proteins or by silver-stained LPS gels, thus providing evidence that they were of the same strain and that antigenic variation had occurred in vivo.

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