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The Journal of Medical Microbiology, Vol 35, Issue 5 297-303, Copyright © 1991 by Society for General Microbiology

JOURNAL ARTICLE

Restriction endonuclease digest patterns of chromosomal DNA from group B beta-haemolytic streptococci

Y. Nagano, N. Nagano, S. Takahashi, K. Murono, K. Fujita, F. Taguchi and Y. Okuwaki
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Sagamihara, Japan.

Scanning densitometry and computer-assisted numerical analysis were used to examine restriction endonuclease digest patterns (RDPs) of chromosomal DNA from 26 infecting strains and 44 vaginal isolates of group B beta-haemolytic streptococci (GBS). At the 95% similarity level, HindIII RDPs of serotype Ia and III strains clustered into four and three RDP types, respectively. Nine of 10 strains from neonates with early-onset septicaemia belonged to two particular RDP types (Ia-3 and III-3). In contrast, serotype III GBS strains from meningitis cases were not characterised by particular RDP types. Associations between RDPs and certain phenotypic characteristics were also found.


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