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The Journal of Medical Microbiology, Vol 35, Issue 3 152-158, Copyright © 1991 by Society for General Microbiology

JOURNAL ARTICLE

A comparison of probes for restriction fragment length polymorphism (RFLP) typing of Legionella pneumophila serogroup 1 strains

N. A. Saunders, T. G. Harrison, A. Haththotuwa and A. G. Taylor
Division of Microbiological Reagents and Quality Control, Central Public Laboratory, London.

The use of probes derived from rRNA sequences to detect restriction fragment length polymorphisms (RFLPs) associated with the ribosomal RNA genes for epidemiological typing (ribotyping) is a powerful and readily applicable tool. Different probes and enzymes for ribotyping were compared for a series of 73 unrelated Legionella pneumophila serogroup 1 strains. The probes compared were cDNAs, transcribed from L. pneumophila or Escherichia coli rRNA subunits, and a cloned L. pneumophila rRNA gene. The cloned rRNA gene probe gave the best discrimination and this probe was further compared with cloned probes comprised of randomly selected (non-rRNA) parts of the L. pneumophila chromosome. In this instance the greatest discrimination was achieved when one of the non-ribosomal RNA gene probes was employed. The overall discrimination of RFLP typing was enhanced by combining the data obtained with both rRNA and non-rRNA probes.


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B. S. Fields, R. F. Benson, and R. E. Besser
Legionella and Legionnaires' Disease: 25 Years of Investigation
Clin. Microbiol. Rev., July 1, 2002; 15(3): 506 - 526.
[Abstract] [Full Text] [PDF]


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