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The Journal of Medical Microbiology, Vol 34, Issue 3 175-180, Copyright © 1991 by Society for General Microbiology

JOURNAL ARTICLE

Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella

A. Cloeckaert, I. Jacques, N. Bosseray, J. N. Limet, R. Bowden, G. Dubray and M. Plommet
Unit of Experimental Medicine, Catholic University of Louvain, Brussels, Belgium.

Twenty-six monoclonal antibodies (MAbs) directed against seven brucella outer-membrane proteins (OMPs) of 10, 16.5, 19, 25-27, 31-34, 36-38 and 89 Kda were screened for passive protection of mice; three MAbs (directed against 16.5, 25-27 and 36-38 Kda) reduced significantly the initial colonisation of the spleen measured 7 days after challenge. Although significant, the reduction in numbers of Brucella organisms in the spleen was low compared with that conferred by MAbs against lipopolysaccharide of smooth specificity (S-LPS). The three most protective MAbs belonged to two isotypes (IgG1 and IgG2a) and were specific for three different OMPs. No relationship between protection and binding of MAbs to the challenge S strain or its rough mutant was observed in the mouse model. The humoral protection depended mainly on antibodies directed against S-LPS, although some MAbs against OMPs had weak protective activity.


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