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The Journal of Medical Microbiology, Vol 34, Issue 1 39-43, Copyright © 1991 by Society for General Microbiology

JOURNAL ARTICLE

Detection of HSV-1 DNA in patients with Behcet's syndrome and in patients with recurrent oral ulcers by the polymerase chain reaction

M. Studd, D. J. McCance and T. Lehner
Department of Immunology, United Medical School Guy's Hospital, London.

The polymerase chain reaction was used to detect HSV-1 DNA in genomic DNA extracted from peripheral blood leucocytes, in patients with Behcet's syndrome (BS), patients with recurrent oral ulcers and normal healthy controls. A 211-bp HSV-1 DNA fragment was found in a significant number of patients with BS (p less than 0.02). Serum anti-HSV-1 antibodies were also found in a higher proportion of patients with BS (p less than 0.02) than in healthy controls. However, virus-specific DNA was not detected in biopsy samples taken from oral ulcers in patients with BS.


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