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Protein profile typing—a new method of typing Morganella morganii strains

Department of Medical Microbiology, Dundee University Medical School, Ninewells Hospital, Dundee DD1 9SY

^* Institute of Microbiology, University Medical School, H-7643 Pécs, Hungary

Received March 22, 1990
Accepted May 21, 1990

Surmmary: A new, simple and stable method for typing Morganella morganii strains is described. The 150 strains examined, principally from faeces, contained haemolytic and non-haemolytic representatives of diverse O serogroup, bacteriocin type and biotype. Among the biotypes were some trehalose-fermenting, tetracycline-resistant strains and some non-motile, tetracycline-sensitive, glycerol fermenters. After analysis of cell lysates by sodium dodecyl sulphate-discontinuous polyacrylamide gel electrophoresis, strains could be differentiated into 21 types on the basis of outer membrane proteins (OMP) of 35–40 Kda. The OMP profile was not altered by culture on various common media and was unrelated to either O antigen or morganocin p-type. The finest strain recognition in M. morganii can be achieved by application of all three distinct typing methods.