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J Med Microbiol 32 (1990), 39-43; DOI: 10.1099/00222615-32-1-39
© 1990 Society for General Microbiology
ISSN 0022-2615
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Cytolytic action of Vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity

H. YAMANAKA, K. SUGIYAMA*, H. FURUTA*, S. MIYOSHI and S. SHINODA

Department of Environmental Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-Naka, Okayama 700

* Department of Pharmacology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700, Japan

Received May 15, 1989 Revision received October 30, 1989.
The mode of action of Vibrio vulnificus haemolysin (VVH) on mast cells from the peritoneal cavity of the rat was examined. VVH induced histamine release, and damage to the mast cells, in a dose-dependent fashion. When 1 µUg of VVH was added to c. 105 mast cells at 37°C, histamine release was observed after a lag period of 5–10 s, and was complete within 5 min. The action was temperature-dependent, and was not induced at 4°C. Disodium cromoglycate, a membrane stabiliser for mast cells, inhibited the histamine release significantly, but the effect was not dose-dependent. Moreover, leakage of lactate dehydrogenase from VVH-treated mast cells was observed. These results suggest that VVH acts on the cell membrane of mast cells and is cytolytic.




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