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The Journal of Medical Microbiology, Vol 31, Issue 4 241-249, Copyright © 1990 by Society for General Microbiology

JOURNAL ARTICLE

Plasmid and chromosomal related toxin polymorphism of Escherichia coli serogroup O 138; plasmid transfer and co-integration with pRP4

M. J. Woodward, C. Wray, G. A. Ridha and J. R. Walton
Department of Bacteriology, MAFF Central Veterinary Laboratory, New Haw, Weybridge, Surrey.

DNA-DNA hybridisation was used to examine 89 isolates of Escherichia coli serogroup O138 for toxin gene content and location. Sixty-six isolates encoded toxin genes; 29 were STpa STpb VT2, 24 were VT2, four were STpa STpb, three were STpb VT2, three were STpb VT2LT and one was STpa. None were K88-, K99- or F41-positive. The VT toxin gene was chromosomally located in all VT+ isolates tested whereas STpa, STpb and LT were plasmid borne. In co-transfer experiments with pRP4 as the mobilising plasmid, VT2 genes were not transferred to recipient E. coli K12 whereas STpa, STpb and LT genes were. Two VT2-positive isolates inhibited pRP4 transfer to K12 by a factor of 10(6) and rare transconjugants harboured a co-integrate plasmid comprising pRP4 (54kb) and a cryptic host plasmid (145 kb). In a K12 background, the co-integrate had transfer and incompatibility properties of pRP4 whereas in both progenitor E. coli O138 backgrounds the co-integrate was transfer inhibited and in one case resolved to give original plasmids.




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