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The Journal of Medical Microbiology, Vol 31, Issue 2 85-93, Copyright © 1990 by Society for General Microbiology

JOURNAL ARTICLE

A comparison of specificity and biological activity of polyclonal and monoclonal antibodies raised against Salmonella minnesota R595 lipopolysaccharide

N. D. Mehta, B. M. Wilson, N. T. Rapson and C. S. Easmon
Department of Hematology/Oncology, Michael Reese Hospital and Medical Center, Chicago, IL.

Murine monoclonal antibodies (MAbs) and immune rabbit serum were raised against the rough mutant of Salmonella minnesota strain R595. These antibodies were tested for their ability to inhibit LPS-induced B-cell mitogenicity and neutralise LPS toxicity in chick embryos. Immune rabbit serum inhibited both mitogenicity and LPS lethality. None of the MAbs or a cocktail of antibodies were able to neutralise LPS lethality in chick embryos. However, they were able to inhibit mitogenicity by varying degrees.




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